Protein Knockout Platform

Both RNAi and protein knockout technologies are able to reduce the steady state levels of cellular proteins of interest. Specifically, RNAi operates at the level of protein biosynthesis via degrading specific mRNA or inhibiting its translation, protein removal from the cell, however, solely relies on the endogenous protein destruction machinery to remove the protein; whereas the proprietary protein knockout technology functions at the post-translational level to accelerate the degradation of the desired protein, but won't stop new protein synthesis. Our integrated RNAi and protein knockout system therefore significantly improves the efficiency and kinetics of knocking down cellular proteins of interest, providing an excellent platform for protein function interrogation. This integrated technology is especially advantageous when manipulating proteins with a long half-life.

We have degradation vectors targeting several key cellular proteins involved in cell growth control and tumorigensis, such as retinoblastoma protein, cytosol β–catenin, and p107 tumor suppressor.

Potential Applications

• Kits for sale to the academic and commercial research markets
• Contract research, especially validating targets for pharma/biotech
• Possibly, development of drug candidates